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anti mef2d  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc anti mef2d
    Anti Mef2d, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 93/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+mef2d/MEF2D+Rabbit+mAb/pm41131393-385-14-15
    Average 93 stars, based on 4 article reviews
    anti mef2d - by Bioz Stars, 2026-09
    93/100 stars

    Images

    Related Articles

    Western Blot:

    Article Title: The nuclear receptor, Nor-1, markedly increases type II oxidative muscle fibers and resistance to fatigue.
    Article Snippet: .. Blots were probed with anti-Nor-1 (1:300; Santa Cruz Biotechnology, Inc., Santa Cruz, CA), MitoProfile Total OXPHOS Rodent WB Antibody cocktail (1:500, MS604; MitoSciences, Eugene, OR), anti-HDAC5 (no. 2082, 1:1000; Cell Signaling, Beverly, MA), anti-pHDAC5 (Ser259, ab53693, 1:1000; Abcam, Cambridge, MA), anti-HDAC4 (no. 5392, 1:1000; Cell Signaling), antipHDAC4/5/7 (Ser246/259/155, respectively, no. 3443, 1:1000; Cell Signaling), antiglyceraldehyde-3-phosphate dehydrogenase (GAPDH) (2275-PC-100, 1:20,000; Trevigen, Gaithersburg, MD), anti-MEF2c (Ab64644, 1:1000; Abcam), anti-MEF2d (Ab32845, 1:1000; Abcam), anti-GLUT4 (Ab62375, 1:5000; Abcam), anti-pPKD1 (S738 S742, ab74105, 1:1000; Abcam), anti-AMPK (no. 2532, 1:1000; Cell Signaling), or antipAMPK (Thr172, no. 2535S, 1:1000; Cell Signaling) in Trisbuffered saline with 5% skim milk powder (or 1% BSA for antiphospho-proteins). .. Secondary antirabbit/mouse-horseradish peroxidase (Pierce Biotechnology, Rockford, IL) was used at 1:5000 to 1:10,000 in 5% skim milk powder.

    Article Title: The Nuclear Receptor, Nor-1, Markedly Increases Type II Oxidative Muscle Fibers and Resistance to Fatigue
    Article Snippet: .. Blots were probed with anti- Nor-1 (1:300; Santa Cruz Biotechnology, Inc., Santa Cruz, CA), MitoProfile Total OXPHOS Rodent WB Antibody cocktail (1:500, MS604; MitoSciences, Eugene, OR), anti-HDAC5 (no. 2082, 1:1000; Cell Signaling, Beverly, MA), anti-pHDAC5 (Ser259, ab53693, 1:1000; Abcam, Cambridge, MA), anti-HDAC4 (no. 5392, 1:1000; Cell Signaling), anti-pHDAC4/5/7 (Ser246/259/155, respectively, no. 3443, 1:1000; Cell Signaling), antiglyceraldehyde-3-phosphate dehydrogenase (GAPDH) (2275-PC-100, 1:20,000; Trevigen, Gaithersburg, MD), anti-MEF2c (Ab64644, 1:1000; Abcam), anti-MEF2d (Ab32845, 1:1000; Abcam), anti-GLUT4 (Ab62375, 1:5000; Abcam), anti-pPKD1 (S738+S742, ab74105, 1:1000; Abcam), anti-AMPKα (no. 2532, 1:1000; Cell Signaling), or anti-pAMPKα (Thr172, no. 2535S, 1:1000; Cell Signaling) in Tris-buffered saline with 5% skim milk powder (or 1% BSA for antiphospho-proteins). .. Secondary antirabbit/mouse-horseradish peroxidase (Pierce Biotechnology, Rockford, IL) was used at 1:5000 to 1:10,000 in 5% skim milk powder.

    Saline:

    Article Title: The nuclear receptor, Nor-1, markedly increases type II oxidative muscle fibers and resistance to fatigue.
    Article Snippet: .. Blots were probed with anti-Nor-1 (1:300; Santa Cruz Biotechnology, Inc., Santa Cruz, CA), MitoProfile Total OXPHOS Rodent WB Antibody cocktail (1:500, MS604; MitoSciences, Eugene, OR), anti-HDAC5 (no. 2082, 1:1000; Cell Signaling, Beverly, MA), anti-pHDAC5 (Ser259, ab53693, 1:1000; Abcam, Cambridge, MA), anti-HDAC4 (no. 5392, 1:1000; Cell Signaling), antipHDAC4/5/7 (Ser246/259/155, respectively, no. 3443, 1:1000; Cell Signaling), antiglyceraldehyde-3-phosphate dehydrogenase (GAPDH) (2275-PC-100, 1:20,000; Trevigen, Gaithersburg, MD), anti-MEF2c (Ab64644, 1:1000; Abcam), anti-MEF2d (Ab32845, 1:1000; Abcam), anti-GLUT4 (Ab62375, 1:5000; Abcam), anti-pPKD1 (S738 S742, ab74105, 1:1000; Abcam), anti-AMPK (no. 2532, 1:1000; Cell Signaling), or antipAMPK (Thr172, no. 2535S, 1:1000; Cell Signaling) in Trisbuffered saline with 5% skim milk powder (or 1% BSA for antiphospho-proteins). .. Secondary antirabbit/mouse-horseradish peroxidase (Pierce Biotechnology, Rockford, IL) was used at 1:5000 to 1:10,000 in 5% skim milk powder.

    Article Title: The Nuclear Receptor, Nor-1, Markedly Increases Type II Oxidative Muscle Fibers and Resistance to Fatigue
    Article Snippet: .. Blots were probed with anti- Nor-1 (1:300; Santa Cruz Biotechnology, Inc., Santa Cruz, CA), MitoProfile Total OXPHOS Rodent WB Antibody cocktail (1:500, MS604; MitoSciences, Eugene, OR), anti-HDAC5 (no. 2082, 1:1000; Cell Signaling, Beverly, MA), anti-pHDAC5 (Ser259, ab53693, 1:1000; Abcam, Cambridge, MA), anti-HDAC4 (no. 5392, 1:1000; Cell Signaling), anti-pHDAC4/5/7 (Ser246/259/155, respectively, no. 3443, 1:1000; Cell Signaling), antiglyceraldehyde-3-phosphate dehydrogenase (GAPDH) (2275-PC-100, 1:20,000; Trevigen, Gaithersburg, MD), anti-MEF2c (Ab64644, 1:1000; Abcam), anti-MEF2d (Ab32845, 1:1000; Abcam), anti-GLUT4 (Ab62375, 1:5000; Abcam), anti-pPKD1 (S738+S742, ab74105, 1:1000; Abcam), anti-AMPKα (no. 2532, 1:1000; Cell Signaling), or anti-pAMPKα (Thr172, no. 2535S, 1:1000; Cell Signaling) in Tris-buffered saline with 5% skim milk powder (or 1% BSA for antiphospho-proteins). .. Secondary antirabbit/mouse-horseradish peroxidase (Pierce Biotechnology, Rockford, IL) was used at 1:5000 to 1:10,000 in 5% skim milk powder.

    Incubation:

    Article Title: MEF2D-expressing cancer precursors reprogram tissue-resident macrophages to support liver tumorigenesis.
    Article Snippet: Cancers often originate from precursor cells within an inflamed microenvironment; however, the mechanisms by which these precursors manipulate the niche to promote tumorigenesis remain unclear.. By combining single-cell and spatial transcriptomic analyses of precancerous lesions in hepatocellular carcinoma, here we show that elevated myocyte enhancer factor 2D (MEF2D)-expressing cancer precursors reprogram liver-resident macrophages, Kupffer cells (KCs), to create a growth-supportive environment.. MEF2D levels induce an oncogenic and secretory phenotype in these precursors by epigenetic reprogramming, which is crucial for tumor initiation in murine models and human samples.

    Article Title: Atorvastatin reverses cardiac remodeling possibly through regulation of protein kinase D/myocyte enhancer factor 2D activation in spontaneously hypertensive rats.
    Article Snippet: The present study was designed to determine whether atorvastatin reduced hypertension-induced cardiac remodeling and whether these effects involved Protein Kinase D (PKD) and Myocyte Enhancer Factor 2D (MEF2D), factors known to be implicated in cardiac hypertrophy and fibrosis.. 16-Week-old spontaneously hypertensive rats (SHRs) and age-matched Wistar-Kyoto (WKY) rats were included.. Blood pressure and serum lipid concentration were measured.



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    RMS survival is selectively regulated by MyoD (A) Representative images of immunohistochemistry staining of MyoD and myogenin in patient embryonal and alveolar tumors. (B) Histologic images in (A) were scored for MyoD and myogenin immunohistochemistry staining positivity, n = 5. (C) RH30-SR cells expressing a scrambled-control sh-RNA or sh-RNA targeting myogenin were treated with TNF and DOX for 24 h and subsequently measured for cell death, n = 3. (D) RNA was prepared from cells in (C) and qPCR analysis was performed probing for myogenin and its target genes, n = 3. (E) RH30-SR cells expressing a scrambled-control sh-RNA or sh-RNA against either MyoD, MYF5, myogenin, MRF4, MEF2C, and <t>MEF2D</t> (sh-MRFs) were treated with TNF for 24 h and cell death was subsequently measured, n = 3. (F) CHRONOS Score for MRFs in sarcoma cell lines tested in DepMap. RMS cell lines are highlighted in purple, with a score of < −1 indicating a gene that is essential; ATRT, atypical teratoid rhabdoid tumors; ES, Ewing sarcoma; FS, follicular sarcoma; LMS, leiomyosarcoma; LS, liposarcoma; MRT, malignant rhabdoid tumor; PS, pleomorphic sarcoma; RMS, rhabdomyosarcoma; SS, synovial sarcoma; TS, thyroid sarcoma; US, undifferentiated sarcoma. (G and H) Kaplan-Meier curve, log rank test, showing the correlation of both (G) MyoD and (H) myogenin expression stratified by median patient expression to overall survival of rhabdomyosarcoma patients from an R2 Genomics Analysis and Visualization Platform. (I) CHRONOS Score for p65 in sarcoma cell lines tested in the Cancer Dependency Map. Data with error bars are depicted as mean ± SEM, ∗ p < 0.05; ∗∗ p < 0.01. See also .
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    RMS survival is selectively regulated by MyoD (A) Representative images of immunohistochemistry staining of MyoD and myogenin in patient embryonal and alveolar tumors. (B) Histologic images in (A) were scored for MyoD and myogenin immunohistochemistry staining positivity, n = 5. (C) RH30-SR cells expressing a scrambled-control sh-RNA or sh-RNA targeting myogenin were treated with TNF and DOX for 24 h and subsequently measured for cell death, n = 3. (D) RNA was prepared from cells in (C) and qPCR analysis was performed probing for myogenin and its target genes, n = 3. (E) RH30-SR cells expressing a scrambled-control sh-RNA or sh-RNA against either MyoD, MYF5, myogenin, MRF4, MEF2C, and <t>MEF2D</t> (sh-MRFs) were treated with TNF for 24 h and cell death was subsequently measured, n = 3. (F) CHRONOS Score for MRFs in sarcoma cell lines tested in DepMap. RMS cell lines are highlighted in purple, with a score of < −1 indicating a gene that is essential; ATRT, atypical teratoid rhabdoid tumors; ES, Ewing sarcoma; FS, follicular sarcoma; LMS, leiomyosarcoma; LS, liposarcoma; MRT, malignant rhabdoid tumor; PS, pleomorphic sarcoma; RMS, rhabdomyosarcoma; SS, synovial sarcoma; TS, thyroid sarcoma; US, undifferentiated sarcoma. (G and H) Kaplan-Meier curve, log rank test, showing the correlation of both (G) MyoD and (H) myogenin expression stratified by median patient expression to overall survival of rhabdomyosarcoma patients from an R2 Genomics Analysis and Visualization Platform. (I) CHRONOS Score for p65 in sarcoma cell lines tested in the Cancer Dependency Map. Data with error bars are depicted as mean ± SEM, ∗ p < 0.05; ∗∗ p < 0.01. See also .
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    RMS survival is selectively regulated by MyoD (A) Representative images of immunohistochemistry staining of MyoD and myogenin in patient embryonal and alveolar tumors. (B) Histologic images in (A) were scored for MyoD and myogenin immunohistochemistry staining positivity, n = 5. (C) RH30-SR cells expressing a scrambled-control sh-RNA or sh-RNA targeting myogenin were treated with TNF and DOX for 24 h and subsequently measured for cell death, n = 3. (D) RNA was prepared from cells in (C) and qPCR analysis was performed probing for myogenin and its target genes, n = 3. (E) RH30-SR cells expressing a scrambled-control sh-RNA or sh-RNA against either MyoD, MYF5, myogenin, MRF4, MEF2C, and <t>MEF2D</t> (sh-MRFs) were treated with TNF for 24 h and cell death was subsequently measured, n = 3. (F) CHRONOS Score for MRFs in sarcoma cell lines tested in DepMap. RMS cell lines are highlighted in purple, with a score of < −1 indicating a gene that is essential; ATRT, atypical teratoid rhabdoid tumors; ES, Ewing sarcoma; FS, follicular sarcoma; LMS, leiomyosarcoma; LS, liposarcoma; MRT, malignant rhabdoid tumor; PS, pleomorphic sarcoma; RMS, rhabdomyosarcoma; SS, synovial sarcoma; TS, thyroid sarcoma; US, undifferentiated sarcoma. (G and H) Kaplan-Meier curve, log rank test, showing the correlation of both (G) MyoD and (H) myogenin expression stratified by median patient expression to overall survival of rhabdomyosarcoma patients from an R2 Genomics Analysis and Visualization Platform. (I) CHRONOS Score for p65 in sarcoma cell lines tested in the Cancer Dependency Map. Data with error bars are depicted as mean ± SEM, ∗ p < 0.05; ∗∗ p < 0.01. See also .
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    Figure 3 | Adeno-associated virus was injected into the skeletal muscle of C57BL/6 mice in situ (n = 6). (a) Mouse life curve. (b) Body weight of mice in each group. (c) General pictures of mice in each group. (d) The general map and fluorescence intensity of each tissue of mice injected with the adeno-associated virus group were captured by a small animal imager. (e) mCherry protein expression in mouse tissues (skeletal muscle, heart, liver, epididymis white adipose tissue, subcutaneous white adipose tissue and brown adipose tissue). (f–i) Expression of <t>MEF2D</t> and GLUT4 in skeletal muscle tissues of mice in each group. (j, k) Glucose tolerance and area under the curve (AUC) of mice in each group. (l, m) Insulin sensitivity and area under the curve (AUC) of mice in each group. (t-test, *, compared with HFD-NC group, P < 0.05; #, compared with HFD- mimics group, P < 0.05, the difference was statistically significant).
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    Image Search Results


    RMS survival is selectively regulated by MyoD (A) Representative images of immunohistochemistry staining of MyoD and myogenin in patient embryonal and alveolar tumors. (B) Histologic images in (A) were scored for MyoD and myogenin immunohistochemistry staining positivity, n = 5. (C) RH30-SR cells expressing a scrambled-control sh-RNA or sh-RNA targeting myogenin were treated with TNF and DOX for 24 h and subsequently measured for cell death, n = 3. (D) RNA was prepared from cells in (C) and qPCR analysis was performed probing for myogenin and its target genes, n = 3. (E) RH30-SR cells expressing a scrambled-control sh-RNA or sh-RNA against either MyoD, MYF5, myogenin, MRF4, MEF2C, and MEF2D (sh-MRFs) were treated with TNF for 24 h and cell death was subsequently measured, n = 3. (F) CHRONOS Score for MRFs in sarcoma cell lines tested in DepMap. RMS cell lines are highlighted in purple, with a score of < −1 indicating a gene that is essential; ATRT, atypical teratoid rhabdoid tumors; ES, Ewing sarcoma; FS, follicular sarcoma; LMS, leiomyosarcoma; LS, liposarcoma; MRT, malignant rhabdoid tumor; PS, pleomorphic sarcoma; RMS, rhabdomyosarcoma; SS, synovial sarcoma; TS, thyroid sarcoma; US, undifferentiated sarcoma. (G and H) Kaplan-Meier curve, log rank test, showing the correlation of both (G) MyoD and (H) myogenin expression stratified by median patient expression to overall survival of rhabdomyosarcoma patients from an R2 Genomics Analysis and Visualization Platform. (I) CHRONOS Score for p65 in sarcoma cell lines tested in the Cancer Dependency Map. Data with error bars are depicted as mean ± SEM, ∗ p < 0.05; ∗∗ p < 0.01. See also .

    Journal: iScience

    Article Title: MyoD is essential in rhabdomyosarcoma by promoting survival through differentiation and CYLD

    doi: 10.1016/j.isci.2025.113149

    Figure Lengend Snippet: RMS survival is selectively regulated by MyoD (A) Representative images of immunohistochemistry staining of MyoD and myogenin in patient embryonal and alveolar tumors. (B) Histologic images in (A) were scored for MyoD and myogenin immunohistochemistry staining positivity, n = 5. (C) RH30-SR cells expressing a scrambled-control sh-RNA or sh-RNA targeting myogenin were treated with TNF and DOX for 24 h and subsequently measured for cell death, n = 3. (D) RNA was prepared from cells in (C) and qPCR analysis was performed probing for myogenin and its target genes, n = 3. (E) RH30-SR cells expressing a scrambled-control sh-RNA or sh-RNA against either MyoD, MYF5, myogenin, MRF4, MEF2C, and MEF2D (sh-MRFs) were treated with TNF for 24 h and cell death was subsequently measured, n = 3. (F) CHRONOS Score for MRFs in sarcoma cell lines tested in DepMap. RMS cell lines are highlighted in purple, with a score of < −1 indicating a gene that is essential; ATRT, atypical teratoid rhabdoid tumors; ES, Ewing sarcoma; FS, follicular sarcoma; LMS, leiomyosarcoma; LS, liposarcoma; MRT, malignant rhabdoid tumor; PS, pleomorphic sarcoma; RMS, rhabdomyosarcoma; SS, synovial sarcoma; TS, thyroid sarcoma; US, undifferentiated sarcoma. (G and H) Kaplan-Meier curve, log rank test, showing the correlation of both (G) MyoD and (H) myogenin expression stratified by median patient expression to overall survival of rhabdomyosarcoma patients from an R2 Genomics Analysis and Visualization Platform. (I) CHRONOS Score for p65 in sarcoma cell lines tested in the Cancer Dependency Map. Data with error bars are depicted as mean ± SEM, ∗ p < 0.05; ∗∗ p < 0.01. See also .

    Article Snippet: Antibodies used in this study included, IκBα (Cell Signaling Technology Cat# 9242, RRID: AB_331623 ), α-tubulin (Thermo Fisher Scientific Cat# A11126, RRID: AB_2534135 ), p65/RelA (Santa Cruz Biotechnology Cat# sc-109, RRID: AB_632039 ), MyoD (Santa Cruz Biotechnology Cat# sc-377460, RRID: AB_2813894 ), Myf5 (Santa Cruz Biotechnology Cat# sc-518039), myogenin (Santa Cruz Biotechnology Cat# sc-52903, RRID: AB_784707 ), MRF4 (Santa Cruz Biotechnology sc-514379), MEF2C (Abcam Cat# ab211493, RRID: AB_2864417 ), MEF2D (Santa Cruz Biotechnology Cat# sc-271153, RRID: AB_10614669 ), MYHC (Sigma-Aldrich Cat# M8421, RRID: AB_477248 ), TnnT (Sigma-Aldrich Cat# SAB4200717, RRID: AB_2892079 ), CYLD (Cell Signaling Technology Cat# 4495, RRID: AB_10557111 ), p -RIPK1 (Proteintech Cat# 66854-1-Ig, RRID: AB_2882194 ), RIPK1 (Thermo Fisher Scientific Cat# PA5-20811, RRID: AB_11154790 ), DNMT1 (Novus Cat# NB100-56519, RRID: AB_838131 ), DNMT3A (Santa Cruz Biotechnology Cat# sc-365769, RRID: AB_10844010 ), DNMT3B (Cell Signaling Technology Cat# 67259, RRID: AB_2799723 ), and HRP conjugated secondary antibodies anti-mouse IgG (Promega Cat# W4021, RRID: AB_430834 ) and anti-rabbit IgG (Promega Cat# W4011, RRID: AB_430833 ).

    Techniques: Immunohistochemistry, Staining, Expressing, Control

    Figure 3 | Adeno-associated virus was injected into the skeletal muscle of C57BL/6 mice in situ (n = 6). (a) Mouse life curve. (b) Body weight of mice in each group. (c) General pictures of mice in each group. (d) The general map and fluorescence intensity of each tissue of mice injected with the adeno-associated virus group were captured by a small animal imager. (e) mCherry protein expression in mouse tissues (skeletal muscle, heart, liver, epididymis white adipose tissue, subcutaneous white adipose tissue and brown adipose tissue). (f–i) Expression of MEF2D and GLUT4 in skeletal muscle tissues of mice in each group. (j, k) Glucose tolerance and area under the curve (AUC) of mice in each group. (l, m) Insulin sensitivity and area under the curve (AUC) of mice in each group. (t-test, *, compared with HFD-NC group, P < 0.05; #, compared with HFD- mimics group, P < 0.05, the difference was statistically significant).

    Journal: Journal of diabetes investigation

    Article Title: Adipocyte exosome miR-4472 inhibits glucose uptake in skeletal muscle through downregulation of MEF2D.

    doi: 10.1111/jdi.70054

    Figure Lengend Snippet: Figure 3 | Adeno-associated virus was injected into the skeletal muscle of C57BL/6 mice in situ (n = 6). (a) Mouse life curve. (b) Body weight of mice in each group. (c) General pictures of mice in each group. (d) The general map and fluorescence intensity of each tissue of mice injected with the adeno-associated virus group were captured by a small animal imager. (e) mCherry protein expression in mouse tissues (skeletal muscle, heart, liver, epididymis white adipose tissue, subcutaneous white adipose tissue and brown adipose tissue). (f–i) Expression of MEF2D and GLUT4 in skeletal muscle tissues of mice in each group. (j, k) Glucose tolerance and area under the curve (AUC) of mice in each group. (l, m) Insulin sensitivity and area under the curve (AUC) of mice in each group. (t-test, *, compared with HFD-NC group, P < 0.05; #, compared with HFD- mimics group, P < 0.05, the difference was statistically significant).

    Article Snippet: ZSGB-BIO (China) provided the b-actin (ab234430) and GAPDH (TA-08) antibodies, while Proteintech (China) provided the MEF2D (PA5-30950) and GLUT4 (66846-1-Ig) antibodies.

    Techniques: Virus, Injection, In Situ, Fluorescence, Expressing