anti mef2d (Cell Signaling Technology Inc)
Structured Review
Anti Mef2d, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 93/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+mef2d/MEF2D+Rabbit+mAb/pm41131393-385-14-15
Average 93 stars, based on 4 article reviews
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Western Blot:Article Title: The nuclear receptor, Nor-1, markedly increases type II oxidative muscle fibers and resistance to fatigue. Article Snippet: .. Blots were probed with anti-Nor-1 (1:300; Santa Cruz Biotechnology, Inc., Santa Cruz, CA), MitoProfile Total OXPHOS Rodent WB Antibody cocktail (1:500, MS604; MitoSciences, Eugene, OR), anti-HDAC5 (no. 2082, 1:1000; Cell Signaling, Beverly, MA), anti-pHDAC5 (Ser259, ab53693, 1:1000; Abcam, Cambridge, MA), anti-HDAC4 (no. 5392, 1:1000; Cell Signaling), antipHDAC4/5/7 (Ser246/259/155, respectively, no. 3443, 1:1000; Cell Signaling), antiglyceraldehyde-3-phosphate dehydrogenase (GAPDH) (2275-PC-100, 1:20,000; Trevigen, Gaithersburg, MD), anti-MEF2c (Ab64644, 1:1000; Abcam), anti-MEF2d (Ab32845, 1:1000; Abcam), anti-GLUT4 (Ab62375, 1:5000; Abcam), anti-pPKD1 (S738 S742, ab74105, 1:1000; Abcam), anti-AMPK (no. 2532, 1:1000; Cell Signaling), or antipAMPK (Thr172, no. 2535S, 1:1000; Cell Signaling) in Trisbuffered saline with 5% skim milk powder (or 1% BSA for antiphospho-proteins). .. Secondary antirabbit/mouse-horseradish peroxidase (Pierce Biotechnology, Rockford, IL) was used at 1:5000 to 1:10,000 in 5% skim milk powder. Article Title: The Nuclear Receptor, Nor-1, Markedly Increases Type II Oxidative Muscle Fibers and Resistance to Fatigue Article Snippet: .. Blots were probed with anti- Nor-1 (1:300; Santa Cruz Biotechnology, Inc., Santa Cruz, CA), MitoProfile Total OXPHOS Rodent WB Antibody cocktail (1:500, MS604; MitoSciences, Eugene, OR), anti-HDAC5 (no. 2082, 1:1000; Cell Signaling, Beverly, MA), anti-pHDAC5 (Ser259, ab53693, 1:1000; Abcam, Cambridge, MA), anti-HDAC4 (no. 5392, 1:1000; Cell Signaling), anti-pHDAC4/5/7 (Ser246/259/155, respectively, no. 3443, 1:1000; Cell Signaling), antiglyceraldehyde-3-phosphate dehydrogenase (GAPDH) (2275-PC-100, 1:20,000; Trevigen, Gaithersburg, MD), anti-MEF2c (Ab64644, 1:1000; Abcam), anti-MEF2d (Ab32845, 1:1000; Abcam), anti-GLUT4 (Ab62375, 1:5000; Abcam), anti-pPKD1 (S738+S742, ab74105, 1:1000; Abcam), anti-AMPKα (no. 2532, 1:1000; Cell Signaling), or anti-pAMPKα (Thr172, no. 2535S, 1:1000; Cell Signaling) in Tris-buffered saline with 5% skim milk powder (or 1% BSA for antiphospho-proteins). .. Secondary antirabbit/mouse-horseradish peroxidase (Pierce Biotechnology, Rockford, IL) was used at 1:5000 to 1:10,000 in 5% skim milk powder. Saline:Article Title: The nuclear receptor, Nor-1, markedly increases type II oxidative muscle fibers and resistance to fatigue. Article Snippet: .. Blots were probed with anti-Nor-1 (1:300; Santa Cruz Biotechnology, Inc., Santa Cruz, CA), MitoProfile Total OXPHOS Rodent WB Antibody cocktail (1:500, MS604; MitoSciences, Eugene, OR), anti-HDAC5 (no. 2082, 1:1000; Cell Signaling, Beverly, MA), anti-pHDAC5 (Ser259, ab53693, 1:1000; Abcam, Cambridge, MA), anti-HDAC4 (no. 5392, 1:1000; Cell Signaling), antipHDAC4/5/7 (Ser246/259/155, respectively, no. 3443, 1:1000; Cell Signaling), antiglyceraldehyde-3-phosphate dehydrogenase (GAPDH) (2275-PC-100, 1:20,000; Trevigen, Gaithersburg, MD), anti-MEF2c (Ab64644, 1:1000; Abcam), Article Title: The Nuclear Receptor, Nor-1, Markedly Increases Type II Oxidative Muscle Fibers and Resistance to Fatigue Article Snippet: .. Blots were probed with anti- Nor-1 (1:300; Santa Cruz Biotechnology, Inc., Santa Cruz, CA), MitoProfile Total OXPHOS Rodent WB Antibody cocktail (1:500, MS604; MitoSciences, Eugene, OR), anti-HDAC5 (no. 2082, 1:1000; Cell Signaling, Beverly, MA), anti-pHDAC5 (Ser259, ab53693, 1:1000; Abcam, Cambridge, MA), anti-HDAC4 (no. 5392, 1:1000; Cell Signaling), anti-pHDAC4/5/7 (Ser246/259/155, respectively, no. 3443, 1:1000; Cell Signaling), antiglyceraldehyde-3-phosphate dehydrogenase (GAPDH) (2275-PC-100, 1:20,000; Trevigen, Gaithersburg, MD), anti-MEF2c (Ab64644, 1:1000; Abcam), Incubation:Article Title: MEF2D-expressing cancer precursors reprogram tissue-resident macrophages to support liver tumorigenesis. Article Snippet: Cancers often originate from precursor cells within an inflamed microenvironment; however, the mechanisms by which these precursors manipulate the niche to promote tumorigenesis remain unclear.. By combining single-cell and spatial transcriptomic analyses of precancerous lesions in hepatocellular carcinoma, here we show that elevated myocyte enhancer factor 2D (MEF2D)-expressing cancer precursors reprogram liver-resident macrophages, Kupffer cells (KCs), to create a growth-supportive environment.. MEF2D levels induce an oncogenic and secretory phenotype in these precursors by epigenetic reprogramming, which is crucial for tumor initiation in murine models and human samples. Article Title: Atorvastatin reverses cardiac remodeling possibly through regulation of protein kinase D/myocyte enhancer factor 2D activation in spontaneously hypertensive rats. Article Snippet: The present study was designed to determine whether atorvastatin reduced hypertension-induced cardiac remodeling and whether these effects involved Protein Kinase D (PKD) and Myocyte Enhancer Factor 2D (MEF2D), factors known to be implicated in cardiac hypertrophy and fibrosis.. 16-Week-old spontaneously hypertensive rats (SHRs) and age-matched Wistar-Kyoto (WKY) rats were included.. Blood pressure and serum lipid concentration were measured. |

